Research Compendium/NAD⁺
Anti-Ageing & LongevityResearch OnlyNot For Human Consumption

NAD⁺

Nicotinamide Adenine Dinucleotide · NAD+ injectable

Essential coenzyme that declines ~50% per decade - injectable NAD⁺ restores mitochondrial function, DNA repair, and sirtuin activity faster than oral precursors.

MECHANISM OF ACTION

NAD⁺ (nicotinamide adenine dinucleotide) is a central coenzyme in cellular energy metabolism, serving as an electron carrier in the mitochondrial electron transport chain. Beyond energy production, NAD⁺ is a substrate for sirtuins (SIRT1–7 - longevity regulators), PARP (DNA repair enzymes), and CD38 (immune regulation). NAD⁺ levels decline ~50% per decade, contributing to reduced mitochondrial function, impaired DNA repair, and metabolic decline. Injectable NAD⁺ bypasses oral bioavailability limitations and achieves faster intracellular repletion vs. NMN or NR precursors - making it the preferred format in aggressive research protocols.

RESEARCH APPLICATIONS

  • Mitochondrial bioenergetics restoration
  • Sirtuin activation (SIRT1/3 longevity pathway)
  • DNA repair enhancement (PARP substrate)
  • Cellular energy metabolism and mitochondrial function research
  • Cognitive clarity and neurological function research

RESEARCH HIGHLIGHTS

Sirtuin Activation and Longevity

2013

NAD⁺ repletion in aged mice activated SIRT1 and SIRT3, restoring mitochondrial function, improving muscle endurance, and extending healthspan in multiple studies.

Ref: Gomes et al., Cell

DNA Repair via PARP

2016

NAD⁺ depletion impairs PARP-mediated DNA repair; repletion restores repair capacity - particularly relevant in age-related genomic instability models.

Ref: Fang et al., Cell Metabolism

RESEARCH PROTOCOL NOTES

Injectable formats300–1000mg IV infusion or SC injection
IV noteIV infusion causes flush/tightness at rapid rates - slow infusion over 2–3 hours recommended
Available formatsVial (300–600mg), Pen (1000mg - NAD+ Pen)
vs. NMN/NRInjectable NAD+ achieves faster repletion; NMN/NR are oral precursors converted to NAD+ intracellularly

Chemical Identity

Molecular FormulaC21H27N7O14P2
Molecular Weight663.4 g/mol
CAS Number53-84-9
Half-Life (t½)Very short in plasma - rapidly internalised by cells
SolubilityHighly soluble in water

Storage & Stability

Lyophilised: 2–8°C, 12–24 months. Reconstituted: use within 24–48 hours at 2–8°C. Highly sensitive to light and oxidation - use amber vials, protect from air.

Regulatory Status

Not a registered pharmaceutical. Injectable vials supplied as research compound only. Not WADA prohibited.

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